講演情報
[3Ap11]The evaluation of casein nanoparticles as carrier for astaxanthin: Fabrication, characterization and stability
*Rui xi Yu1、Xuan Wang2、Yu Sun1、Yu Gu2、Ying Zhao1,3 (1. College of Biological Engineering, Henan University of Technology、2. School of International Education,Henan University of Technology、3. Laboratory of Nutritional Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University)
キーワード：
アスタキサンチン、H/J型凝集体、安定性
【Purpose】
Astaxanthin (AST) is a caroteniod with antioxidant and anti-inflammatory properties. However, its application in the food and biomedicine industry was limited due to its poor water dispersibility and chemical stability. Here,casein (Cas) was chosen as a nanocarrier for astaxanthin (AST) and this study explored the interactions between Cas and AST.
【Methods】
H/J-type aggregates Astaxanthin/Casein Nanoparticles (H/J-AC-NPs) were fabricated via a bottom-up layer-by-layer self-assembly nano-technique. The particle sizes of the H/J-AC-NPs were analyzed with DLS. Morphological features of the nanoparticles were imaged by TEM and SEM. This study employed spectroscopic methods such as the UV–visible absorption spectra, fluorescence spectra and fourier transform infrared spectroscopy to investigate the mechanisms.
【Results】
The optimal nanoparticle sizes of H-AC-NPs and J-AC-NPs were 422 nm and 596 nm, respectively. The encapsulation efficiency of AST by Cas reached 79.1% with the load capacity of 4.5%. Spectral analysis showed that hydrophobic interaction,hydrogen bonds and Van der Waals forces in the H/J-AC-NPs. AST binding altered the secondary structure of Cas, with the increase of random coil and β-fold content and the decrease of α-helix content.These findings demonstrated that H/J-AC-NPs significantly improved water solubility and stability of AST and its aggregates，thus providing guidance for highlighting its potential applications in the food and pharmaceutical industries.
Astaxanthin (AST) is a caroteniod with antioxidant and anti-inflammatory properties. However, its application in the food and biomedicine industry was limited due to its poor water dispersibility and chemical stability. Here,casein (Cas) was chosen as a nanocarrier for astaxanthin (AST) and this study explored the interactions between Cas and AST.
【Methods】
H/J-type aggregates Astaxanthin/Casein Nanoparticles (H/J-AC-NPs) were fabricated via a bottom-up layer-by-layer self-assembly nano-technique. The particle sizes of the H/J-AC-NPs were analyzed with DLS. Morphological features of the nanoparticles were imaged by TEM and SEM. This study employed spectroscopic methods such as the UV–visible absorption spectra, fluorescence spectra and fourier transform infrared spectroscopy to investigate the mechanisms.
【Results】
The optimal nanoparticle sizes of H-AC-NPs and J-AC-NPs were 422 nm and 596 nm, respectively. The encapsulation efficiency of AST by Cas reached 79.1% with the load capacity of 4.5%. Spectral analysis showed that hydrophobic interaction,hydrogen bonds and Van der Waals forces in the H/J-AC-NPs. AST binding altered the secondary structure of Cas, with the increase of random coil and β-fold content and the decrease of α-helix content.These findings demonstrated that H/J-AC-NPs significantly improved water solubility and stability of AST and its aggregates，thus providing guidance for highlighting its potential applications in the food and pharmaceutical industries.
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